APA
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Tachibana, S., Chen, C., Zhang, O. R., Schurr, S. V., Hill, C., Li, R., … Cho, Y. (2019). Analyzing Oxygen Consumption Rate in Primary Cultured Mouse Neonatal Cardiomyocytes Using an Extracellular Flux Analyzer. Journal of Visualized Experiments.
Chicago/Turabian
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Tachibana, Shizuko, Chao Chen, Oliver R Zhang, Sarah V. Schurr, C. Hill, Ruixia Li, A. M. Manso, et al. “Analyzing Oxygen Consumption Rate in Primary Cultured Mouse Neonatal Cardiomyocytes Using an Extracellular Flux Analyzer.” Journal of Visualized Experiments (2019).
MLA
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Tachibana, Shizuko, et al. “Analyzing Oxygen Consumption Rate in Primary Cultured Mouse Neonatal Cardiomyocytes Using an Extracellular Flux Analyzer.” Journal of Visualized Experiments, 2019.
BibTeX Click to copy
@article{shizuko2019a,
title = {Analyzing Oxygen Consumption Rate in Primary Cultured Mouse Neonatal Cardiomyocytes Using an Extracellular Flux Analyzer.},
year = {2019},
journal = {Journal of Visualized Experiments},
author = {Tachibana, Shizuko and Chen, Chao and Zhang, Oliver R and Schurr, Sarah V. and Hill, C. and Li, Ruixia and Manso, A. M. and Zhang, Jianlin and Andreyev, A. and Murphy, A. and Ross, R. and Cho, Yoshitake}
}
Mitochondria and oxidative metabolism are critical for maintaining cardiac muscle function. Research has shown that mitochondrial dysfunction is an important contributing factor to impaired cardiac function found in heart failure. By contrast, restoring defective mitochondrial function may have beneficial effects to improve cardiac function in the failing heart. Therefore, studying the regulatory mechanisms and identifying novel regulators for mitochondrial function could provide insight which could be used to develop new therapeutic targets for treating heart disease. Here, cardiac myocyte mitochondrial respiration is analyzed using a unique cell culture system. First, a protocol has been optimized to rapidly isolate and culture high viability neonatal mouse cardiomyocytes. Then, a 96-well format extracellular flux analyzer is used to assess the oxygen consumption rate of these cardiomyocytes. For this protocol, we optimized seeding conditions and demonstrated that neonatal mouse cardiomyocytes oxygen consumption rate can be easily assessed in an extracellular flux analyzer. Finally, we note that our protocol can be applied to a larger culture size and other studies, such as intracellular signaling and contractile function analysis.